The Juventus star flew home early from international duty with Portugal earlier this month after returning a positive test.
Ronaldo has tested positive again and will now miss the Champions League clash with Barca, according to Marca.
However, it is claimed Juventus will ask UEFA for permission to test their talisman 48 hours ahead of the game in a bid to make him available for the reunion with Lionel Messi on October 28.
Ronaldo has been working hard to keep himself fit during isolation and the second positive test will come as a big blow.
The 35-year-old remains upbeat despite his latest setback as he took to social media on Thursday afternoon.
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